Detection of Escherichia coli in water using a colorimetric gene probe assay
Titel:
Detection of Escherichia coli in water using a colorimetric gene probe assay
Auteur:
Rice, Eugene W. Covert, Terry C. Johnson, Scott A. Johnson, Clifford H. Reasoner, Donald J.
Verschenen in:
Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering
Paginering:
Jaargang 30 (1995) nr. 5 pagina's 1059-1067
Jaar:
1995-06
Inhoud:
A commercially available DNA hydribization assay (Gene-trak(R), Framingham, MA, USA) was compared with the EC-MUG procedure for the detection of Escherichia coli in water. The gene probe gave positive responses for pure cultures of E. coli, E. coli 0157:H7, E. fergusonii. Shigella sonnei, S. dysenteriae and S. boydii. The hybridization assay was capable of detecting E. coli in environmental samples and survivors among chlorine exposed cells.