A model in vitro system for assessing the effects of oxidant stressor agents on red cells with chemically-induced superoxide dismutase deficiency
Titel:
A model in vitro system for assessing the effects of oxidant stressor agents on red cells with chemically-induced superoxide dismutase deficiency
Auteur:
Horton, Holly M. Calabrese, Edward J.
Verschenen in:
Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering
Paginering:
Jaargang 21 (1986) nr. 3 pagina's 249-265
Jaar:
1986-04
Inhoud:
An in vitro test system was established to determine the oxidant Stressor effects of various agents on erythrocytes having low Superoxide dismutase (SOD) activity. The compound, diethyldithiocarbamate (DDC) was used to inhibit SOD activity in normal human erythrocytes. SOD activity was reduced to 67%, 50% and 41% of normal via the action of 1 mM, 2 mM and 4mM DDC, respectively. Five environmental agents, carbaryl, copper, nitrite, paraquat and p-chloroaniline, were evaluated. P-chloroaniline (5 mM) and carbaryl (2 mM) caused significantly raised methemoglobin levels (p = .001) in erythrocytes with 41% of normal SOD activity. Linear regression analyses indicated that a significant linear increase in methemoglobin levels (p = .001) occurred for erythrocytes, having reduced SOD activity, incubated with p-chloroaniline and carbaryl.
Tijdschrift beschrijving