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| Titel: |
Deuterated Disaccharides for the Investigation of Protein-Carbohydrate Interactions-Application of Bioaffinity-and STD-NMR |
| Auteur: |
Hellebrandt, Wilfried Haselhorst, Thomas Kohli, Thies Bauml, Englbert Peters, Thomas |
| Verschenen in: |
Journal of carbohydrate chemistry |
| Paginering: | Jaargang 19 (2000) nr. 6 pagina's 769-782 |
| Jaar: |
2000 |
| Inhoud: |
NMR spectroscopic analysis of carbohydrates often suffers from severe overlap of resonance signals, especially in 1H NMR spectra. Therefore, we synthesized four 2,3,4-trideuterio-α-L-fucose containing disaccharides, α-L-Fuc-(1→6)-β-D-GlcNAc-OMe 1, α-L-Fuc-(1→4)-β-D-GlcNAc-OMe 2, α-L-Fuc-(1→3)-β-D-GlcNAc-OMe 3, and α-L-Fuc-(1→2)-β-D-Gal-OMe 4. Compounds 1 to 4 are well suited to be subjected to NMR conformational analysis because their 1H NMR spectra show almost no overlap of signals. The deuterated disaccharides 1 to 4 will therefore be used as NMR probes for the exploration of fucose-binding proteins. With a mixture of the corresponding non-deuterated disaccharides it is demonstrated that recently developed parallel NMR screening protocols, Bio-Affinity NMR and STD-NMR, deliver fast and robust tools to assay the compounds synthesized for protein-binding affinity. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 5 van 9 gevonden artikelen
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