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Details van artikel 20 van 178 gevonden artikelen
| Titel: |
Application of Periodate Oxidized Glucans to Biochemical Reactions |
| Auteur: |
Kobayashi, Mikihiko Ichishima, Eiji |
| Verschenen in: |
Journal of carbohydrate chemistry |
| Paginering: | Jaargang 10 (1991) nr. 4 pagina's 635-644 |
| Jaar: |
1991 |
| Inhoud: |
Periodate oxidation of glucans afforded a dialdehyde structure, which was highly reactive with various compounds containing amino groups. A covalent Schiff base linkage was readily formed at the alkaline pH of 8-9 and cyclodextrin dialdehyde gave both positively and negatively charged derivatives upon incubation with hexamethylenediamine and ɛ-aminocaproic acid, respectively. Binding of the amino group containing a fluorescent probe of ethylenediaminonaphthalene yielded fluorescent glycogen, which was hydrolyzed with Taka-amylase A. By gel filtration with a Bio-Gel P-2 column, hydrolyzed oligosaccharides containing a fluorescent probe were strongly retained to the column. Dextran dialdehyde was useful in producing a covalent linkage with trypsin under very mild conditions, and the enzyme-dextran complex formed was recovered in a high-molecular weight and active form. Thus, various glucan dialdehydes may serve as useful cross-linking reagents for enzymes. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 20 van 178 gevonden artikelen
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