Derivatization Procedures for Reducing Oligosaccharides, Part 4: Use of Glycosylamines in a Reversible Derivatization of Oligosaccharides with the 9-Fluorenylmethoxycarbonyl Group, and Hplc Separations of the Derivatives
Titel:
Derivatization Procedures for Reducing Oligosaccharides, Part 4: Use of Glycosylamines in a Reversible Derivatization of Oligosaccharides with the 9-Fluorenylmethoxycarbonyl Group, and Hplc Separations of the Derivatives
Auteur:
Kallin, Elisabeth Lonn, Hans Norberg, Thomas Sund, Torbjorn Lundqvist, Mons
Verschenen in:
Journal of carbohydrate chemistry
Paginering:
Jaargang 10 (1991) nr. 3 pagina's 377-386
Jaar:
1991
Inhoud:
A new, reversible derivatization procedure for reducing oligosaccharides was developed. Reaction of the model compound lacto-N-tetraose (β-D-Gal-(1→3)-β-D-GlcNAc-(1→3)-β-D-Gal-(1→4)-D-Glc) with aqueous ammonium bicarbonate gave the corresponding β-glycosylamine, which was reacted with fluorenylmethoxycarbonyl chloride to give an N-fluorenyl-methoxycarbonyl β-glycosylamine (FMOC derivative). The free oligosaccharide could be recovered in high yield from the FMOC derivative by brief treatment with 15% aqueous ammonia. Complex oligosaccharide mixtures from human milk were derivatized with the new procedure, and excellent separations of the derivatives on straight-phase silica HPLC columns were achieved.