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Details for article 146 of 252 found articles
| Title: |
Structural Studies on a Cell Wall Polysaccharide Preparation of Lactococcus Lactis Subspecies Cremoris H414 |
| Author: |
Gruter, Marijke Billy, Didier de Waard, Pieter Kuiper, Jan Kamerling, Johannis P. Vliegenthart, Johannes F. G. |
| Appeared in: |
Journal of carbohydrate chemistry |
| Paging: | Volume 13 (1994) nr. 3 pages 363-382 |
| Year: |
1994-04-01 |
| Contents: |
A cell wall polysaccharide preparation of Lactococcus lactis subsp. cremoris H414 was isolated by nitrous acid extraction, and purified by gel filtration and anion-exchange chromatography. Oligosaccharide fragments, obtained by partial acid hydrolysis or alkaline hydrolysis, were studied by means of monosaccharide analysis, methylation analysis, FAB-MS, EI-MS, and 1H NMR spectroscopy. In addition 1H, 13C, and 31P NMR spectroscopy, including two-dimensional homo- and heteronuclear measurements, were performed on the native polysaccharide. Combination of the various results demonstrated that in the polysaccharide preparation two repeating units with the structures [→2)-α-l-Rhap-(1→3)-α-d-Galp-(1→]n and [→6)-[α-d-Galp-(1→4)]-α-d-GalpNAc-(1→3)-β-d-GalpNAc-(1→1)-Ribitol-(4-phosphate→]m, respectively, can be recognized. So far, a covalent linkage between both polymers could not be confirmed nor be excluded. |
| Publisher: |
Taylor & Francis |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 146 of 252 found articles
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