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  Sterilization of Plants for Phytoremediation Studies by Bleach Treatment
 
 
Titel: Sterilization of Plants for Phytoremediation Studies by Bleach Treatment
Auteur: Medina, Victor F.
Jeffers, Peter M.
Larson, Steven L.
Perez, Waleska
Verschenen in: International journal of phytoremediation
Paginering: Jaargang 2 (2000) nr. 4 pagina's 287-295
Jaar: 2000-10-01
Inhoud: Bleach treatment of plants was studied as a simple alternative to axenic tissue cultures for demonstrating phytodegradation of aqueous and gas-phase environmental contaminants. Parrotfeather (Myriophyllum aquaticum), spinach (Spinacia oleracea), and wheat (Triticum aestivum) were exposed to 0.525% NaC10 solutions for 15 s, then rinsed in deionized water. Plate counts indicated that 97 to 100% of viable bacteria were removed from parrotfeather and spinach. Transformation rates for 2,4,6-trinitrotoluene (TNT) by bleached and untreated parrotfeather were virtually identical. Similarly, treated and untreated spinach, wheat heads, and wheat leaves removed methyl bromide (MeBr) from air at the same rates. However, wheat root with attendant adhering soil was rendered inactive by bleach treatment. Parrotfeather roots examined by dissecting microscope and by electron microscope showed no significant damage caused by bleach treatment.
Uitgever: Taylor & Francis
Bronbestand: Elektronische Wetenschappelijke Tijdschriften
 
 

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