Dyes and dusts have been used to mark insects internally and externally for decades, the majority of examples coming from laboratory-reared pest species used in mark-release-recapture studies. Using dyes or dusts to mark populations of pests and beneficial insects simultaneously in the field has received less attention. We evaluated a water-soluble fluorescent dye and a resin-based fluorescent pigment sprayed on crops to mark beneficial and pest insects, and monitored the dispersal of marked insects. Our results show that resin-based dyes provide an effective mark on several species of insects among several orders. The resin-based dye is also relatively inexpensive, non-toxic, UV-stable and water resistant, unlike a water-soluble dye. Using the resin-based dye in a broccoli production system, we were able to monitor simultaneously the movement of field populations of the parasitoids, Diadegma semiclausum (Hellen) (Hymenoptera: Ichneumonidae), and Apanteles ippeus (Nixon) (Hymenoptera: Braconidae) and the adult stage of the host, diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae). Resin-based dye applied on a crop is an effective way to mark and monitor the dispersal of populations of beneficial and pest insects in relation to agricultural practices, integrated pest management and conservation biological control.