Comparison of two glutathione S-transferases used in capacitive biosensors for detection of heavy metals
Titel:
Comparison of two glutathione S-transferases used in capacitive biosensors for detection of heavy metals
Auteur:
SaatCI, Ebru Nistor, Mihaela Gaspar, Szilveszter Csoregi, Elisabeth Iscan, Mesude
Verschenen in:
International journal of environmental analytical chemistry
Paginering:
Jaargang 87 (2007) nr. 10-11 pagina's 745-754
Jaar:
2007-08
Inhoud:
This work describes the development of a heavy-metal biosensor based on either recombinant 6His-Tag glutathione S-transferase (GST-(His)6) or glutathione S-transferase Theta 2-2 (GST-theta 2-2), and a capacitive transducer. The dynamic range of the pure bovine liver GST-Theta 2-2 biosensor was 1 fM to 1 mM for Zn2+, and 10 pM to 1 mM for Cd2+. The GST-(His)6 biosensor was able to detect Zn2+ and Cd2+ in the range of 1 fM to 10 µM, and Hg2+ in the range of 1 fM to 10 mM. The bovine liver GST Theta 2-2 biosensor displays an increased selectivity and a wider dynamic range for Zn2+ compared with the GST-(His)6 biosensor. Therefore, by using different GST isozymes, it is possible to modulate important characteristics of capacitive biosensors for the detection of heavy metals.