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| Titel: |
Production and characterization of group-specific antibodies against penicillin antibiotics |
| Auteur: |
Usleber, Ewald Litz, Susanne Martlbauer, Erwin |
| Verschenen in: |
Food & agricultural immunology |
| Paginering: | Jaargang 10 (1998) nr. 4 pagina's 317-324 |
| Jaar: |
1998-12 |
| Inhoud: |
Polyclonal antibodies against penicillin antibiotics were produced in rabbits after immunization with an ampicillin-bovine serum albumin conjugate. The antibodies were tested in a competitive direct enzyme immunoassay (EIA) using an ampicillin-horseradish peroxidase conjugate as the labelled antigen and penicillin G as the reference antibiotic. The 50% inhibition concentration for penicillin G was 12 ng ml-1. This penicillin EIA showed broad group-specificity for penicillins having the intact 6-aminopenicillanic acid (6-APA) nucleus, and was able to detect 16 other penicillins tested, whereas cephalosporin antibiotics were not detected. Relative cross-reactivities (penicillin G = 100%) exceeding 10% were found for amoxicillin (45%), ampicillin (45%), 6-8 (12%), azlocillin (110%), carbenicillin (39%), cloxacillin (49%), dicloxacillin (57%), epicillin (23%), metampicillin (130%), methicillin (32%), oxacillin (98%), penicillin V (160%), pheneticillin (68%), piperacillin (26%) and ticarcillin (110%). After alkaline hydrolysis of the ss-lactam ring, the corresponding penicilloyl derivatives of these antibiotics completely lost cross-reactivity in the test system. Therefore, the antibodies described here primarily detect the intact 6-APA nucleus having any carboxylamide side chain. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 8 van 8 gevonden artikelen
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