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| Titel: |
Characterization of the interactions between immobilized parathion and the corresponding recombinant scfv antibody using a piezoelectric biosensor |
| Auteur: |
Horacek, Jiri Garrett, Stephen D. Skladal, Petr Morgan, Michael R. A. |
| Verschenen in: |
Food & agricultural immunology |
| Paginering: | Jaargang 10 (1998) nr. 4 pagina's 363-374 |
| Jaar: |
1998-12 |
| Inhoud: |
A piezoelectric quartz crystal sensor with immobilized parathion was used for real-time kinetic characterization of the interactions with recombinant anti-parathion single chain Fv antibody IFRN AA01 (scFv). Parathion was linked to the sensor gold electrodes modified with a self-assembled layer of aminothiophenol using either bovine serum albumin (BSA) or dextran as spacer molecules. The kinetic dissociation rate constant kd was 8 × 10-4 s-1 for both types of sensors, and the association rate constants ka, were 590 and 260 mol-1 1 s-1 for BSA and dextran-linked parathion, respectively. The regeneration of BSA-parathion coated crystals was not successful, however. Those crystals with bound scFv were used to study the formation of scFv dimers. Dextran-parathion modified crystals were successfully regenerated using proteinase K. The affinity of scFv to dextran-parathion was 50 X lower when compared with the affinity of the anti-parathion monoclonal antibody (IgG, IFRN 1701) the sequence of which served for production of scFv. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 2 van 8 gevonden artikelen
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