|
| << vorige | volgende >> |
Tijdschrift beschrijving
Alle jaargangen van het bijbehorende tijdschrift
Alle afleveringen van het bijbehorende jaargang
Alle artikelen van de bijbehorende aflevering
Details van artikel 97 van 109 gevonden artikelen
| Titel: |
Rapid screening of fruit juices and water supplies for contamination by 2,4-d using an enzyme immunoassay |
| Auteur: |
Mcmorris, C. J. McConnell, R. I. Lamont, J. V. Fitzgerald, S. P. |
| Verschenen in: |
Food & agricultural immunology |
| Paginering: | Jaargang 6 (1994) nr. 3 pagina's 261-266 |
| Jaar: |
1994 |
| Inhoud: |
An enzyme-linked immunosorbent assay (ELISA) for 2,4-dichlorophenoxy acetic acid (2,4-D) was developed using polyclonal antibodies raised in sheep, immunized with a derivative of 2,4-D (2,4-dichlorophenoxy acetyl-2-succinamyl hydrazine) conjugated to keyhole limpet haemocyanin. The mean percentage recovery of 2,4-D from fortified water samples was 104% and ranged from 96 to 120% for fortified orange juice. The sensitivity of the assay without concentration of sample or sample clean-up was 0.2 ng ml -1 for water and 50 ng ml -1 for orange juice. With dichloromethane extraction of 2,4-D from orange juice, 1 ng ml -1 could be detected. 2,4,5-T was the only herbicide investigated which cross-reacted significantly with the 2,4-D antisera. The ELISA also detected 2,4,5-T in water, being sensitive to 0-66 ng ml -1. The ELISA is suitable for direct screening of water and fruit juice samples for 2,4-D contamination. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 97 van 109 gevonden artikelen
| << vorige | volgende >> |