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| Titel: |
An enzyme-linked immunosorbent assay for the direct analysis of beta-agonist drugs in urine and sera |
| Auteur: |
Oriundi, M. Paleologo Giacomini, G. Ballaben, F. Berti, F. Benedetti, F. Bagnati, R. Bastiani, E. |
| Verschenen in: |
Food & agricultural immunology |
| Paginering: | Jaargang 4 (1992) nr. 2 pagina's 73-82 |
| Jaar: |
1992 |
| Inhoud: |
A sensitive competitive solid-phase enzyme immunoassay for the detection of tert-butylic beta-agonist drugs was developed. An antiserum was raised in rabbits against the diazo-conjugate of clenbuterol and the human serum albumin. In the assay, the anticlenbuterol antibody was incubated with the salbutamol-enzyme conjugate and unlabelled standard or samples in microtitre wells precoated with affinity-purified antirabbit IgG. The absolute detection limit for clenbuterol and mabuterol was 0.3 ng/ml (15 pg/well) and 50% relative binding at 1.0 ng/ml (50 pg/well). The absolute detection limits for salbutamol and terbutaline were 0.9 and 1.3 ng/ml, respectively. The cross-reactivity of iso-propylic parent compounds was quite low (<1%). Urine and sera were tested without any extraction step. Sample blanks (96 urine and 76 sera from five different sources) showed low matrix effect, suggesting a limit of decision for bovine urine of 0.6 ng/ml clenbuterol equivalent. Mean recoveries of clenbuterol in spiked urine and serum samples ranged from 90 to 120% (mean recovery 96%). Comparative analysis by gas chromatography/mass spectrometry showed that false negative results do not occur. The assay is simple, rapid, sensitive, multiresidual, reliable and cost-effective. The test is adaptable to high sample throughput laboratories as well as field test for rapid screening of a few samples in slaughterhouses. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 7 van 135 gevonden artikelen
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