Effect of acid soil chemical properties on nodulation and competition of Rhizobium trifolii
Titel:
Effect of acid soil chemical properties on nodulation and competition of Rhizobium trifolii
Auteur:
Wright, S. F. Wright, R. J. Sworobuk, J. E. Boyer, D. G.
Verschenen in:
Communications in soil science and plant analysis
Paginering:
Jaargang 19 (1988) nr. 3 pagina's 311-325
Jaar:
1988-02
Inhoud:
Soils in the Appalachian region are acidic and many have chemical properties which may inhibit the initiation of clover-Rhizobium leguminosarum biovar trifolii symbiosis. The influence of soil properties on nodulation by, and competition between, an introduced strain (162X95) and a naturalized strain (WV22) of Rhizobium trifolii were studied in limed and unlimed portions of 10 subsoil horizons. Rhizobial strains were inoculated alone or in a 1:1 mixture in liquid form on germinating subterranean clover (Trifolium subterraneum L.). Nodulation by both strains was inhibited in soils with a combination of toxic properties: pH (1:1 H2O) 4.37 to 4.46, Ca saturation 1.1 to 9.2 %, and Al saturation 54.2 to 82.6 %. In the competition experiment strain 162X95 was detected in nodules by enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody. Soil and soil solution chemical properties which were correlated with percent nodule occupancy by strain 162X95 were soil pH (1:1 H2O), soil solution pH, Al saturation, exchangeable Al, soil solution total Al, and soil solution reactive Al (as measured by 8-hydroxyquinoline) (p ≤0.05). Soil pH gave the best estimate of nodule occupancy by strain 162X95 using a second order polynomial: % nodule occupancy = -481.40 + 176.05 soil pH - 14.28 soil pH2 (r2 = 0.62**).