The joint action of mercury and lead on the discharge of antennule-inner ramus nerve of crayfish (Cambarus clarkii) has been studied by means of a multichannel record of electrophysiological signals. the results show: 1. When the concentration of mercury (Hg2+) in water is only half of the restricted concentration (0.1 ug1-1) but there is also 12.5 ug1--1 lead (Pb2+) present, the joint inhibition of the two heavy metallic ions on the discharge is much stronger than that of the restricted concentration of mercury present. the slope of a linear regressive equation which indicates the frequency of discharges (Fn/F1) and action time is < -0.08129 ± 0.00357 ms. Further, in a certain concentration range, the higher the concentration of lead (Pb2+), the inhibition is stronger. 2. When the concentration of lead (Pb2+) in water is only 1/2 of the restricted level (100 ug 1-1Pb2+), but when there is 0.025 ug1-1 Hg2+ present, the joint inhibition of the two heavy metal ions on the discharge is much stronger than the restricted level of lead. the slope of a linear regression indicates a frequency of discharge (Fn/F1) and action time is <-0.07732 ± 0.00451 ms. Within a certain concentration range, the value is higher with a higher concentration of mercury. The results suggest that there are possibly enhanced effects of the toxicity of mercury and lead together. More attention might be made of this in the evaluation of water quality.