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| Titel: |
PRODUCTIVITY ENHANCEMENT OF POLY-(β-HYDROXYBUTYRATE) BY FED-BATCH CULTIVATION OF NUTRIENTS USING VARIABLE (DECREASING) NUTRIENT RATE BY Wautersia eutropha |
| Auteur: |
Khanna, Shilpi Srivastava, Ashok K. |
| Verschenen in: |
Chemical engineering communications |
| Paginering: | Jaargang 195 (2008) nr. 11 pagina's 1424-1436 |
| Jaar: |
2008-11 |
| Inhoud: |
Batch cultivation of Wautersia eutropha carried out at constant pH 7 and temperature 30°C produced a total biomass of 19.7 g/L with a PHB concentration of 10.9 g/L in 60 h. In order to increase the growth and PHB accumulation, a mathematical model was developed and used for offline simulation for feeding of limiting nutrients (carbon and nitrogen). One such cultivation strategy, giving the best biomass and product accumulation at significantly high productivity, was experimentally implemented. The reactor was operated as batch for the first 13 h, after which it was converted to fed-batch by feeding a medium containing 12 g/L nitrogen and 360 g/L fructose at a rate of 100 mL/h. After every 2 h, the rate of the inflowing medium was reduced by 20 mL/h. Nutrient feeding was continued for 8 h. The strategy resulted in 36.2 g/L biomass with an accumulation of 16.8 g/L PHB (productivity of 0.48 g/L-h)in 35 h as opposed to 19.7 g/L biomass and 10.9 g/L PHB in 60 h (productivity of 0.18 g/L-h) in batch cultivation, resulting in a 2.6-fold improvement in productivity. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 11 van 14 gevonden artikelen
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