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Details van artikel 3 van 9 gevonden artikelen
| Titel: |
DESIGN OF RECOMBINANT PROTEIN PRODUCTION PROCESSES |
| Auteur: |
Galvanauskas, Vytautas Volk, Norbert Simutis, Rimvydas Lubbert, Andreas |
| Verschenen in: |
Chemical engineering communications |
| Paginering: | Jaargang 191 (2004) nr. 5 pagina's 732-748 |
| Jaar: |
2004-05 |
| Inhoud: |
A model-aided process design and supervision technique is proposed. Its efficiency is demonstrated by a nontrivial example, the development of a production process for the light chain of the antibody MAK33. This protein was expressed by the genetically modified E. coli B pUBS520 p12023 bacteria under the control of the tac promoter where lactose was used for induction. It is shown in this example that data from a few experiments are sufficient to develop a satisfactory production process. The development of process models, which are prerequisite for a systematic optimization of protein production processes, as well as their use for the determination of quasi-optimal feed-forward control profiles, is discussed. Model-supported closed-loop control is shown to lead to another improvement. Further performance enhancements can be obtained by changing the operational mode from the usually employed fed-batch procedure to a repeated fed-batch mode. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 3 van 9 gevonden artikelen
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