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                                       Details van artikel 3 van 9 gevonden artikelen
 
 
  DESIGN OF RECOMBINANT PROTEIN PRODUCTION PROCESSES
 
 
Titel: DESIGN OF RECOMBINANT PROTEIN PRODUCTION PROCESSES
Auteur: Galvanauskas, Vytautas
Volk, Norbert
Simutis, Rimvydas
Lubbert, Andreas
Verschenen in: Chemical engineering communications
Paginering: Jaargang 191 (2004) nr. 5 pagina's 732-748
Jaar: 2004-05
Inhoud: A model-aided process design and supervision technique is proposed. Its efficiency is demonstrated by a nontrivial example, the development of a production process for the light chain of the antibody MAK33. This protein was expressed by the genetically modified E. coli B pUBS520 p12023 bacteria under the control of the tac promoter where lactose was used for induction. It is shown in this example that data from a few experiments are sufficient to develop a satisfactory production process. The development of process models, which are prerequisite for a systematic optimization of protein production processes, as well as their use for the determination of quasi-optimal feed-forward control profiles, is discussed. Model-supported closed-loop control is shown to lead to another improvement. Further performance enhancements can be obtained by changing the operational mode from the usually employed fed-batch procedure to a repeated fed-batch mode.
Uitgever: Taylor & Francis
Bronbestand: Elektronische Wetenschappelijke Tijdschriften
 
 

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