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Details van artikel 4 van 13 gevonden artikelen
| Titel: |
CHARACTERIZATION OF PROTEINS DURING AGGREGATION II: USE OF MODEL MOLECULES FOR SPECTROSCOPY ANALYSIS |
| Auteur: |
Garcia-Rubio†, L. H. Lopez-Menacho, C. A. Grossman, S. |
| Verschenen in: |
Chemical engineering communications |
| Paginering: | Jaargang 122 (1993) nr. 1 pagina's 85-101 |
| Jaar: |
1993-04-01 |
| Inhoud: |
This study describes the progress made in the use of combined light scattering-spectral deconvolution techniques for the quantitative characterization of proteins in particular, pituitary and recombinant porcine somatotropin. Model molecules for the main ultraviolet chromophores contained in proteins (i.e., tyrosine, tryptophan, phenylalanine and cysteine) have been investigated as functions of ionic strength and denaturant concentration. The objective, in the use of model molecules for the representation of the spectra, is the identification and quantification of the spectral changes undergone by proteins during denaturation and renaturation reactions. Since the denaturation-renaturation reactions of porcine somatotropin involve aggregation processes, the effects of the combined absorption and scattering have been accounted for through the use of Mie and Rayleigh-Gans theories. It is demonstrated that quantitative information on the functional groups participating in the denaturation-renaturation reactions can be obtained through the use of combined light scattering-sped ral deconvolution techniques. The results suggest that not only quantitative size and composition information can be extracted from spectral data but, also, that an effective internal pH for the aggregates may be obtained. The aggregate size and the fraction of dissociated tyrosines estimated suggest that porcine somatotropin may not completely denatured in 6M guanidine/HCl. It is evident that if the appropriate corrections are not included, standard light scattering and uv-vis spectroscopy techniques may be misleading when used for the assessment of the denaturation-renaturation processes. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 4 van 13 gevonden artikelen
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