Histochemical demonstration of enzymes in the uropygial gland of the fowl
Titel:
Histochemical demonstration of enzymes in the uropygial gland of the fowl
Auteur:
Ishida, K. Kusuhara, S. Suzuki, T. Yamaguchi, M.
Verschenen in:
British poultry science
Paginering:
Jaargang 14 (1973) nr. 2 pagina's 179-183
Jaar:
1973-03
Inhoud:
From the distribution of enzymes, the alveoli of a uropygial gland may be divided into inner and outer zones ; the former being the region near the cavity, and the latter the peripheral region of the gland. In the inner zone, there appeared strong activities of 17β-hydroxy-steroid dehydrogenase (17β-OH-SDH), 30α-OH-SDH, glucose-6-phosphate dehydrogenase (G-6-PDH), lactate dehydrogenase (LDH), NAD-dependent malate dehydrogenase (MDH) and acid phosphatase (Acid Pase) weak activities of succinate dehydrogenase (SuDH), NADP-dependent MDH, monoamine oxidase (MAO) and non-specific esterase (Etase), but no activity of β-hydroxybutyrate dehydrogenase (β-HbDH). In the outer zone, there appeared strong activities of LDH and MDH (NAD), moderate activities of 17β-OH-SDH, 3α-OH-SDH, G-6-PDH and β-HbDH, weak activities of SuDH, MDH (NADP) and MAO, but no activities of Etase and Acid Pase. The results were negative concerning ?5-3β-OH-SDH, glutamate dehydrogenase, a-glycerophosphate dehydrogenase, phosphorylase and alkaline phosphatase in both zones. From these results, it was concluded that there must be functional differences between the zones, and that only a part of the metabolic pathways of steroids is being performed.