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| Titel: |
Effects of cycloheximide and changes in lighting regimen on the diurnal rhythm of rat liver protein synthesis |
| Auteur: |
Krug, H. F. Hardeland, R. |
| Verschenen in: |
Biological rhythm research |
| Paginering: | Jaargang 15 (1984) nr. 2 pagina's 119-132 |
| Jaar: |
1984-06 |
| Inhoud: |
Under standard conditions we have demonstrated a highly significant diurnal rhythmicity of translation for both total proteins and integral membrane proteins (IMPs) using a cell-free system from rat liver. IMPs were prepared from microsomes by treatment with EDTA and 2 M LiCl. Normal rhythmic protein synthesis of untreated rats reaches a maximum at 03.00 h and an intermediate maximum at 12.00 h for both total proteins and IMPs. In the following experiments the animals were treated with cycloheximide (CHX) or were subjected to altered lighting regimens before hepatic protein synthesis was investigated in the in vitro system. Injection of CHX 4 hours prior to sacrificing the rats resulted in a marked modification of rhythmic translation, although CHX was washed out from the microsomal preparations and was not present during incubation. The maximal rate of translation was shifted from 03.00 h to 06.00 h, and the intermediate maximum was suppressed. The temporal shift of the maximum after CHX treatment was detected in total proteins, total IMPs, and individual IMPs separated by SDS-polyacrylamide gel electrophoresis. Comparable results were obtained for in vitro protein synthesis after alterations of the lighting regimen. The pattern of overall translation was considerably changed. Dark pulses during light-time stimulated the incorporation, whereas light pulses during dark-time suppressed the normal maximum. Similar results were seen in total as well as in the majority of individual IMPs. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 4 van 10 gevonden artikelen
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