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| Titel: |
Daily rhythms of nuclear protein fractions in rat liver |
| Auteur: |
Beckmann, J. Rensing, L. |
| Verschenen in: |
Biological rhythm research |
| Paginering: | Jaargang 10 (1979) nr. 4 pagina's 297-313 |
| Jaar: |
1979-12 |
| Inhoud: |
Daily changes of a number of nuclear functions of rat liver were analysed in rats kept in a light-dark (LD 12 : 12) cycle and constant temperature. Measurements of the DNA content of rat liver with diphenylamin revealed a mean value of about 3 mg/g liver freshweight without showing significant daily rhy thmicity. When related to mg DNA, no significant rhythmicity could be observed in the total protein content and only a slight rhythmicity in the nuclear protein content. Injection of cycloheximide(2mg/100gbody weight) 10 h before killing the animals resulted in an about 10-20% decrease of the protein content of the tissue as well as of the nucleus and probably in a loss of cell water. Nuclear proteins were separated into nuclear sap proteins, chromatin proteins and restproteins, the first 2 fractions of which were further fractionated by means of polyacrylamide SDS electrophoresis. Considerable differences in the protein content of some of the bands were observed: some bands appeared only at a certain time of day (at 6 h), other bands showed a high amplitude rhythmicity with a maximum at 18 h, whereas other bands— as for example the histone containing bands— varied only slightly during 24 h. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 3 van 5 gevonden artikelen
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