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Details van artikel 5 van 9 gevonden artikelen
| Titel: |
Multipurpose laminar-flow adhesion cells for the study of bacterial colonization and biofilm formation |
| Auteur: |
Mittelman, Marc W. Kohring, Lisa L. White, David C. |
| Verschenen in: |
Biofouling |
| Paginering: | Jaargang 6 (1992) nr. 1 pagina's 39-51 |
| Jaar: |
1992-07 |
| Inhoud: |
The ability to reproducibly colonize substrata with concomitant monitoring of biofilm development is essential to laboratory studies of microbial activities at surfaces. A test system was developed whereby on-line, non-destructive measurements (open circuit potential [OCP], direct cell counts, bioluminescence, oxygen) of bacterial colonization and metabolic activity could be obtained from a series of laminar-flow adhesion cells. The cells consisted of two high-density polyethylene blocks 32 mm H × 65 mm W × 178 mm L, with a 1 mm deep flow channel milled in the top block. A glass viewing window enabled direct observation of a removable, flush-mounted 25 x 50 mm coupon, which was recessed into the bottom block. Laminar-flow conditions were validated at linear flow rates up to 1.3 cm s-1 (20 ml min-1). Reproducible colonization of Pseudomonas fluorescens monocultures was obtained, with 72 h direct-counts and viable counts ranging from between 5.1 to 10.4 × 107 and 1.4 to 2.2 × 107 cells cm-2, respectively, for replicated flow cells (n=20). In situ pulse-labeling of intact biofilms with 14C-acetate resulted in reproducible incorporation of the radiolabel into cell membrane lipids, with 60 min uptake values ranging from 1.0 to 1.8 × 10-5 DPM cell-1. On-line OCP measurements remained stable in sterile test systems, varying by less than 12 mV, for over 80 h. The flow cells provided a means for reproducibly colonizing various substrata under in situ environmental conditions without perturbing the developing biofilms. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 5 van 9 gevonden artikelen
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