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| Titel: |
The surface ultrastructure and adhesive properties of a fimbriate streptococcus sanguis strain and six non-fimbriate mutants |
| Auteur: |
Harty, D. W. S. Willcox, M. D. P. Wyatt, J. E. Oyston, P. C. F. Handley, P. S. |
| Verschenen in: |
Biofouling |
| Paginering: | Jaargang 2 (1990) nr. 1 pagina's 75-86 |
| Jaar: |
1990-02 |
| Inhoud: |
Streptococcus sanguis FW213 carries peritrichous fimbriae (216±28 nm long) and 6 mutants derived from it lack fimbriae but carry peritrichous fibrils with a mean length of 77-4 + 3-9 nm. Both wild type strain and mutants have a ruthenium red staining layer (≤ 14.5±2.9 nm thick) external to the cell wall at the base of the fibrils and fimbriae. The thickness of this layer is strain dependent. Ruthenium red also stains extracellular masses of material, probably extracellular polysaccharide, but not the fimbriae. S. sanguis strain FW 213 adheres to saliva-coated hydroxyapatite and buccal epithelial cells and is not aggregated by saliva. The 6 non-fimbriate mutants of FW213 adhered poorly to hydroxyapatite coated in heated whole saliva (S-SHA) but 3/6 mutants adhered to the same extent or higher than the wild type to S-SHA coated in unheated saliva, indicating that strain FW213 may carry a non-fimbriate adhesin and that whole saliva contains a heat sensitive adhesin. All the mutants had a significantly thinner ruthenium red staining layer (RRL) external to the cell wall than the wild type strain FW213, while the cell surface hydrophobicity showed that the mutants were all less hydrophobic than the wild type FW213. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 10 van 10 gevonden artikelen
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