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| Titel: |
The Development of a Bioluminescence Assay to Compare the Efficacy of Biocides Incorporated into Plasticised PVC |
| Auteur: |
Jones, Catherine Robson, Geoffrey D. Greenhaulgh, Malcolm Eastwood, Ian Handley, Pauline S. |
| Verschenen in: |
Biofouling |
| Paginering: | Jaargang 18 (2002) nr. 1 pagina's 21-27 |
| Jaar: |
2002 |
| Inhoud: |
A bioluminescence assay was developed using the expression of the luxAB genes in Pseudomonas veronii to allow the efficacy of biocides incorporated into plasticised polyvinylchloride (pPVC) to be determined in situ. A maximum number of cells was found to adhere to the surface after 18 h as measured by bioluminescence, radiolabelling and viable cell counts. A positive correlation was found between the level of bioluminescence and numbers of viable cells attached to the pPVC. When the biocide 10, 10-oxybisphenoxyarsine (OBPA) was incorporated into the pPVC, both bioluminescence and viable cell number were reduced by ca 60% at a concentration of 750 ppm and by >99% at 2250 ppm. When the biocide 2,3,5,6-tetrachloro-4-(methylsulphonyl)pyridine (TCMP) was incorporated into the pPVC, no reduction in viability or bioluminescence was seen after 18 h. However, over a period of 72 h at a concentration of 2250 ppm TCMP, both viable cell number and bioluminescence decreased steadily after 36 h until after 72 h, both bioluminescence and viable cell counts were less than 1% of the initial values. The viability of attached cells can therefore be measured in situ in a sensitive real-time assay by measuring bioluminescence allowing the efficacy of biocides incorporated into plastics to be compared. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 9 van 10 gevonden artikelen
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