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| Titel: |
Adsorption of bacterial capsular exopolymers to hydrophilic and hydrophobic surfaces: A 2D-FTIR analysis |
| Auteur: |
Halsall, J. F. Kalaji, M. Neal, A. L. |
| Verschenen in: |
Biofouling |
| Paginering: | Jaargang 16 (2000) nr. 2-4 pagina's 105-118 |
| Jaar: |
2000-11 |
| Inhoud: |
Analysis of the adsorption of capsular exopolymers (EPS) from Pseudomonas sp. NCIMB 2021 to hydrophilic and hydrophobic gold surfaces was examined, in situ, using Fourier transform infrared spectroscopy. The molecular sequence of events occurring upon EPS adsorption to hydrophilic and hydrophobic surfaces has been elucidated using dynamic 2D-FTIR correlation spectroscopy. This method of analysis enables the enhancement of the resolution of overlapping spectral features and the elucidation of time-dependent changes. The data reveal the existence of surface dependent adsorption mechanisms. At both surfaces, the aromatic tyrosyl side chains of the protein moiety displace water. This is followed by an adsorption step dominated by carboxylate groups. However, at the hydrophobic surface, the two steps are interrupted by the ingress of water back to the surface. Furthermore, the amount of neutral exopolymer present was greater at the hydrophilic surface than the hydrophobic surface. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 2 van 27 gevonden artikelen
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