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| Titel: |
Cell surface physicochemistry alters biofilm development of pseudomonas aeruginosa lipopolysaccharide mutants |
| Auteur: |
Flemming, C. A. Palmer, R. J. Arrage, A. A. van der Mei, H. C. White, D. C. |
| Verschenen in: |
Biofouling |
| Paginering: | Jaargang 13 (1998) nr. 3 pagina's 213-231 |
| Jaar: |
1998-12 |
| Inhoud: |
The hydrophobic and electrostatic characteristics of bacterial cell surfaces were compared with attachment proclivity and biomass accumulation over time between wildtype Pseudomonas aeruginosa serotype O6 (possesses A and B band LPS), and three LPS-deficient mutants, viz. A28 (A+B-), R5 (A+B-), and Gt700 (A-B-). The hydrophobic character of each serotype was determined by hydrophobic interaction chromatography and salt-aggregation, and strains were ranked similarly by each method, viz. R5 ≥ A28 > Gt700 > 06. The anionic characteristics of cell-surfaces were determined by electrostatic interaction chromatography and by zeta-potential measurements, and ranked R5 > A28 ≥ Gt700 > 06. Adhesion and biofilm accumulation on stainless steel were significantly different between strains, following the order R5 > A28 >> O6>Gt700. Biofilm rankings were similar on glass, a second hydrophilic substratum. The mutant strains with a strongly hydrophobic character (R5 and A28) demonstrated a significantly greater capacity to form biofilms. These adherent mutants also appeared to have a more anionic cell surface, which may have played a role in biofilm formation on the hydrophilic substrata. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
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