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  Cell surface physicochemistry alters biofilm development of pseudomonas aeruginosa lipopolysaccharide mutants
 
 
Title: Cell surface physicochemistry alters biofilm development of pseudomonas aeruginosa lipopolysaccharide mutants
Author: Flemming, C. A.
Palmer, R. J.
Arrage, A. A.
van der Mei, H. C.
White, D. C.
Appeared in: Biofouling
Paging: Volume 13 (1998) nr. 3 pages 213-231
Year: 1998-12
Contents: The hydrophobic and electrostatic characteristics of bacterial cell surfaces were compared with attachment proclivity and biomass accumulation over time between wildtype Pseudomonas aeruginosa serotype O6 (possesses A and B band LPS), and three LPS-deficient mutants, viz. A28 (A+B-), R5 (A+B-), and Gt700 (A-B-). The hydrophobic character of each serotype was determined by hydrophobic interaction chromatography and salt-aggregation, and strains were ranked similarly by each method, viz. R5 ≥ A28 > Gt700 > 06. The anionic characteristics of cell-surfaces were determined by electrostatic interaction chromatography and by zeta-potential measurements, and ranked R5 > A28 ≥ Gt700 > 06. Adhesion and biofilm accumulation on stainless steel were significantly different between strains, following the order R5 > A28 >> O6>Gt700. Biofilm rankings were similar on glass, a second hydrophilic substratum. The mutant strains with a strongly hydrophobic character (R5 and A28) demonstrated a significantly greater capacity to form biofilms. These adherent mutants also appeared to have a more anionic cell surface, which may have played a role in biofilm formation on the hydrophilic substrata.
Publisher: Taylor & Francis
Source file: Elektronische Wetenschappelijke Tijdschriften
 
 

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