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| Titel: |
Shelf-life of a Biocontrol Pseudomonas putida Applied to Sugar Beet Seeds Using Commercial Coatings |
| Auteur: |
Shah-Smith, D. A. Burns, R. G. |
| Verschenen in: |
Biocontrol science and technology |
| Paginering: | Jaargang 7 (1997) nr. 1 pagina's 65-74 |
| Jaar: |
1997-03-01 |
| Inhoud: |
Pseudomonas putida 40RNF is a putative biological control agent (BCA) of Pythium damping-off of sugar beet. The survival of 40RNF during commercial seed treatment and its subsequent shelf-life (i.e. long-term viability and biocontrol activity) were assessed. Two methods were used to apply 40RNF to sugar beet seeds: incorporation into film-coats sprayed on to pre-pelleted seeds and incorporation into the pellet material prior to pelleting. Only 7.1% of applied 40RNF survived film-coating, but an initial concentration of 7 × 108 ensured that 83.3% of a pre-determined target rate of 6 × 107 |pellet was achieved. After 52 weeks of storage at 4°C,the numbers of 40RNF had declined by one to two orders of magnitude, with a decrease of approximately 50% in disease control. After 52 weeks at 18-20°C, 40RNF was below detectable limits (< 100|pellet), yet the biocontrol activity of the seed treatments was not reduced. The survival of 40RNF during incorporation into the pellet material was poor (< 0.2% of those applied, i.e. 5 × 105 pellet). However, bacterial viability and biocontrol efficacy were maintained at 100% of the control value for 24 weeks when stored at 18-20°C. The results indicate that commercial seed treatments and the storage of pellets at ambient temperatures has potential for the introduction of bacterial BCAs into the spermosphere. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 12 van 14 gevonden artikelen
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