Screening Selected Fungi for Antagonism towards Pseudocercosporella herpotrichoides (Fron) Deighton, the Cause of Eyespot Disease of Cereals
Titel:
Screening Selected Fungi for Antagonism towards Pseudocercosporella herpotrichoides (Fron) Deighton, the Cause of Eyespot Disease of Cereals
Auteur:
Hinton, Melanie J. Parry, D. W.
Verschenen in:
Biocontrol science and technology
Paginering:
Jaargang 3 (1993) nr. 1 pagina's 13-19
Jaar:
1993
Inhoud:
In the laboratory, a dual culture primary screen using wheat straw agar (WSA) showed that 64 out of 152 selected fungi inhibited the in vitro growth of Pseudocercosporella herpotrichoides by 50% or more. An 'asymmetric' species of Penicillium isolated from wheat stubble was the most effective, inhibiting growth of the pathogen by 70%. The results were similar using both W-type and R-type isolates of P. herpotrichoides. Other fungi showing in vitro antagonism included: Trichoderma viride, T. koningii, T. harzianum, Chaetomium globosum, Acremonium persicum, Botryotrichum piluliferum, Sordaria alcina, Microdochium bolleyi, Rhizoctonia cerealis and Laetisaria arvalis. In a glasshouse secondary screen, the 64 fungi showing antagonism in vitro were assessed further for their ability to reduce eyespot disease symptoms of wheat seedlings using a straw collar co-inoculation technique. Of them, 13 fungi reduced disease symptoms significantly: a Chaetomium sp. from stubble, two Fusarium spp., a F. culmorum isolate T. viride, T. koningii, T. harzianum, B. piluliferum, M. bolleyi, L. arvalis, the 'asymmetric' Penicillium sp. and two unidentified fungi from stubble. A F. culmorum isolate was the most effective antagonist on average, giving disease reductions of 54 and 58% for the W-type and R-type of P. herpotrichoides respectively. M. bolleyi, T. harzianum and the F. culmorum isolate were the most effective antagonists of the W-type, all three reducing disease by 54%. T. viride was most effective against the R-type, reducing disease by 64%.
Tijdschrift beschrijving