Deleterious effect of soil-applied metalaxyl and mancozeb on the mycoparasite Pythium oligandrum
Titel:
Deleterious effect of soil-applied metalaxyl and mancozeb on the mycoparasite Pythium oligandrum
Auteur:
White, J. G. Wakeham, A. J. Petch, G. M.
Verschenen in:
Biocontrol science and technology
Paginering:
Jaargang 2 (1992) nr. 4 pagina's 335-340
Jaar:
1992
Inhoud:
Pythium oligandrum was recovered, identified and quantified from air-dried soil plated on 1.5% water agar containing 0.1% glucose. Isolations of P. oligandrum over 2 years from soils treated with single applications of metalaxyl plus mancozeb were consistently lower than those from untreated soil from the same fields. In three fields in the first year P. oligandrum was reduced from a range of 43.3-115.0 to 17.0-43.2 isolates g-1 soil. In the second year, results from 11 fields showed reductions from 27.8-141.8 to 2.8-44.5 isolates g-1 soil. P. oligandrum was sensitive to both metalaxyl and mancozeb, with median effective dose (ED50) values of 0.13 ± 0.02 μg m-1 and 3.33 ± 0.12 μg ml-1, respectively. In a pot test with three soils treated with metalaxyl, mancozeb or the combination of fungicides, levels of P. oligandrum declined over 3 months, with effects first recorded 2 weeks after treatment. Levels of P. oligandrum were reducd by differing degrees in the three soils. Isolate counts from untreated soils declined from a mean of 58.0 g-1 soil at the start of the experiment to 27.5 g-1 after 3 months, whereas fungicide treatments caused further reductions to 11.1 (metalaxyl), 9.7 (mancozeb) and 4.8 isolates g-1 (metalaxyl plus mancozeb).