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| Titel: |
Production of Chlamydospores and Conidia in Submerged Culture by Rhynchosporium alismatis, a Mycoherbicide of Alismataceae in Rice Crops |
| Auteur: |
Cliquet, Sophie Ash, Gavin Cother, Eric |
| Verschenen in: |
Biocontrol science and technology |
| Paginering: | Jaargang 14 (2004) nr. 8 pagina's 801-810 |
| Jaar: |
2004-12 |
| Inhoud: |
The objective of this work was to evaluate the production of chlamydospores and conidia of Rhynchosporium alismatis in a liquid Czapex-Dox based medium supplemented with increasing concentrations of sodium nitrate and malt extract. In addition, the germination of chlamydospores was evaluated. A high concentration of malt extract (4.4 g L-1) as the sole carbon source and a high level of sodium nitrate as the sole nitrogen source (3.3 g L-1) were shown to increase chlamydospore production while agitation (150 rpm) enhanced conidial yields. Maximum chlamydospore production (2.03×105±0.7 total chlamydospores mg DW-1) was achieved in cultures grown in a medium supplemented with 8.8 g L-1 malt extract and 5.74 g L-1 sodium nitrate. Two days growth was required for maximum chlamydospore and conidial production, while 6 days was necessary to obtain maximum dry weight accumulation (350 mg per flask). Germination of chlamydospores (90%) was significantly higher than germination of conidia (47%) after 2 days growth. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 8 van 10 gevonden artikelen
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