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| Titel: |
Monitoring of Biocontrol Agents Based on Trichoderma Strains Following Their Application to Glasshouse Crops by Combining Dilution Plating with UP-PCR Fingerprinting |
| Auteur: |
Lubeck, Mette Jensen, Dan Funck |
| Verschenen in: |
Biocontrol science and technology |
| Paginering: | Jaargang 12 (2002) nr. 3 pagina's 371-380 |
| Jaar: |
2002-06-01 |
| Inhoud: |
Universally primed (UP) PCR analysis was used to characterize strains of Trichoderma spp., which constitute the active ingredients of commercial products for biocontrol of phytopathogens. Several UP primers were able to generate distinct and reproducible fingerprints for each strain, allowing them to be differentiated from a collection of other Trichoderma spp. strains. In order to test whether the UP-PCR method in combination with dilution plating could be useful to detect and enumerate propagules of biocontrol strains of Trichoderma spp. when applied on a commercial scale, sampling was carried out in three commercial glasshouse operations. Dilution plating was carried out using a semi-selective medium and a number of the Trichoderma spp. recovered were subjected to UP-PCR analysis with one selected UP primer. The results showed that the method could be used to identify the isolates from the biocontrol products applied in the different glasshouses. Furthermore, it was found that a biocontrol strain also colonized an untreated bench in one of the glasshouses. The combined methods enable verification of the active ingredient concentration, facilitating monitoring establishment and spread of the Trichoderma strains. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 4 van 10 gevonden artikelen
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