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| Titel: |
Serial propagation and purification of chicken Anaemia agent in mdcc-MSB1 cell line |
| Auteur: |
Goryo, M. Suwa, T. Matsumoto, S. Umemura, T. Itakura, C. |
| Verschenen in: |
Avian pathology |
| Paginering: | Jaargang 16 (1987) nr. 1 pagina's 149-163 |
| Jaar: |
1987 |
| Inhoud: |
The TK-5803 strain of an agent which induces chicken anaemia was propagated and titrated in cultures of the MDCC-MSB1 cell line. When the isolates (tentatively designated MSBI-TK-5803 strain) from the original material were inoculated into 1-day-old susceptible chicks, they showed a severe pathogenicity, inducing anaemia with aplasia of the bone marrow and atrophy of the lymphoid organs. On density gradient separation, the peak of their infectivity titre appeared at a density of 1.35 to 1.36 g/cm3 and numerous virus particles were demonstrated in the same fraction. The agent was resistant to heating for 30 min at 60°C and 50% chloroform for 15 min at 4°C, and passed through a 25 nm membrane filter, but it was completely inactivated after heating for 30 min at 70°C or more. The purified virus particles were spherical or hexagonal in shape and 19.1 ± 0.2 nm in diameter. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 15 van 20 gevonden artikelen
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