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                                       Details for article 7 of 15 found articles
 
 
  Enzyme-linked immunosorbent assay for detection of antibody to leucocytozoon cauller yi
 
 
Title: Enzyme-linked immunosorbent assay for detection of antibody to leucocytozoon cauller yi
Author: Isobe, Takashi
Suzuki, Kyo
Appeared in: Avian pathology
Paging: Volume 15 (1986) nr. 2 pages 199-211
Year: 1986
Contents: An enzyme-linked immunosorbent assay (ELISA) was used to detect antibodies to Leucocytozoon caulleryi in chicken sera. The assay was standardised in terms of antigen coating conditions, optimum antigen concentration, serum and conjugate dilutions. The schizont antigen showed a strong affinity for binding or absorbing to the surface of the polystyrene wells of the microplate. Nonspecific binding of antigen and cross-reactions were not observed with sera from specific-pathogen-free chickens and antisera against various chicken disease agents. The ELISA was more sensitive than the indirect immuno-fluorescent antibody test and the agar gel precipitation test.
Publisher: Taylor & Francis
Source file: Elektronische Wetenschappelijke Tijdschriften
 
 

                             Details for article 7 of 15 found articles
 
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