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Details van artikel 13 van 21 gevonden artikelen
| Titel: | |
| Auteur: | |
| Verschenen in: |
Analytical letters |
| Paginering: | Jaargang 40 (2007) nr. 7 pagina's 1461-1472 |
| Jaar: |
2007-01 |
| Inhoud: |
New polyclonal (pAb) and monoclonal antibodies (mAb) for the immunochemical detection of the anabolic steroids 17β-boldenone and methylboldenone have been developed. Enzyme-linked immunosorbent assays (ELISA) were established for the effective control of these illegally applied growth promoters. The IC50 for methylboldenone of pAb 53 was determined 1.69 ng/l providing a linear measuring range in the ELISA between 0.23 and 12.47 ng/l. The IC50 for methylboldenone of pAb 52 was calculated to be 0.54 µg/l with a linear measuring range between 0.03 and 9.54 µg/l. The mAb Bak detects a minimum concentration of 0.13 µg/l 17β-boldenone. The IC50 corresponds to 1.6 µg/l. The lower detection limit of mAb Zak was determined at 0.3 µg/l 17β-boldenone. The IC50 for this antibody is about 1.2 µg/l. Sample measurements in urine matrices spiked with 2, 3, and 10 µg/l steroid revealed appropriate precision (average CV for pAb of 4.3%, and for mAb of 10.7%) and recovery rates for the developed ELISAs. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 13 van 21 gevonden artikelen
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