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Details van artikel 11 van 18 gevonden artikelen
| Titel: | |
| Auteur: | |
| Verschenen in: |
Analytical letters |
| Paginering: | Jaargang 19 (1986) nr. 5-6 pagina's 639-648 |
| Jaar: |
1986 |
| Inhoud: |
An improved procedure for the determination of caffeine in the presence of bupivicaine (internal standard) using gas liquid chromatography with nitrogen phosphorous detection is described. The method is based on the extraction of caffeine from plasma with a mixture of chloroform and isopropanol (95:5). The chloroform and isopropanol mixture is evaporated to dryness and the residue dissolved in 500 μl of ethyl acetate. One to 2 μl samples are injected directly into the gas chromatograph. This extraction process doesn't give rise to troublesome interfering peaks in the chromatogram. The recovery of caffeine from plasma and breast milk is approximately 99.7% and 94.1% respectively. The coefficient variation of the assay from plasma and breast milk is 2.90% and 1.18% respectively. The limit of quantitation is 0.05 mcg/ml of plasma or breast milk. Data are presented to illustrate the practicality of the method for bioavailability and pharmacokinetic evaluation of caffeine plasma and breast milk levels after oral administration of 100 mg of caffeine to lactating mothers. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 11 van 18 gevonden artikelen
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