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Details for article 9 of 9 found articles
| Title: |
Use of 15N-amino acid isotope dilution techniques to determine endogenous amino acids in ileal digesta in growing pigs |
| Author: |
Gabert, Vince M. Canibe, Nuria Jørgensen, Henry Eggum, Bjørn O. Sauer, Willem C. |
| Appeared in: |
Acta agriculturae Scandinavica. Section A, Animal science |
| Paging: | Volume 47 (1997) nr. 3 pages 168-177 |
| Year: |
1997-08 |
| Contents: |
An experiment was carried out to determine the contribution of endogenous amino acids (AA) to total AA, using the 15N-AA and 15N-leucine isotope dilution techniques, in ileal digesta from growing pigs. Four barrows, initial body weight (BW) 33.8 ± 1.0 kg, were fitted with a simple T-cannula at the distal ileum and one catheter in each of the external jugular veins. The pigs were fed 1.65 kg day-1 of a wheat, fish meal and casein-based diet, formulated to contain 180 g protein (N x 6.25) day kg-1. 15N-leucine, dissolved in saline, was infused into the right catheter at a rate of approximately 40 mg kg BW-1 day-1 for a total of 9 days. The contribution (%) of endogenous AA, which ranged from 17.0 to 84.5%, to total AA in ileal digesta was determined from the dilution of 15N-enrichment (atom percent excess) in the individual AA in digesta compared with free AA in blood plasma. In addition, the dilution of 15N-enrichment in leucine in digesta compared with free leucine in blood plasma was used to predict the endogenous contribution of the other AA, which ranged from 13.9 to 36.0%, and protein (17.4%). The endogenous output of protein was 7.59 g kg dry matter intake (DMI)-1. Endogenous AA outputs ranged from 0.13 to 2.79 g kg DMI-1 and real ileal digestibilities, calculated by correcting apparent digestibilities for endogenous losses, ranged from 73.2 to 97.6%. In conclusion, 15N-amino acid isotope dilution techniques may estimate accurately the endogenous contribution of protein and AA to total protein and AA in ileal digesta. |
| Publisher: |
Taylor & Francis |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 9 of 9 found articles
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