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| Titel: |
Methodological and Functional Aspects of the Isolated Bovine Rumen Epithelium in Ussing Chamber Flux Studies |
| Auteur: |
Sehested, Jakob Diernaes, Lars Laverty, Gary Møller, Peter Detlef Skadhauge, Erik |
| Verschenen in: |
Acta agriculturae Scandinavica. Section A, Animal science |
| Paginering: | Jaargang 46 (1996) nr. 2 pagina's 76-86 |
| Jaar: |
1996-05-01 |
| Inhoud: |
The properties of isolated sheets of bovine rumen epithelium in the Ussing chamber system were studied. The viability and time-dependent change in performance, as expressed by electrical parameters, was acceptable and comparable to what has been obtained with other epithelia in vitro. The viability and time-dependent change in performance was not influenced by transport time (5 to 45 minutes) between the slaughterhouse and the laboratory. Flux rates of 22Na+ and 14C-propionate showed that an equilibration period of 45 minutes after addition of tracers will be sufficient to obtain steady-state conditions with this epithelium. Compared with the time-dependent changes in electrical parameters, this shows that rumen epithelium cannot truly be used as its own control over time. The varying papillation caused surface enlargement factors between one and five, estimated by the dry weight of stripped epithelium. However, the dry weight was not correlated to epithelial capacitance or flux rates. It is concluded that the papillae do not contribute significantly to the fluxes measured in vitro, and that correction for surface enlargement is not essential for the evaluation of flux data. The pH was 0.27 to 0.40 units lower at the epithelial surface than in the bulk solution, and the pH difference was not influenced by glucose or SCFA free solutions, nor by Amiloride (1 mmol 1-1). The observed lower epithelial surface pH seems not to be an acidic microclimate in the traditional sense, but could be explained by a negatively charged surface potential of the epithelial cells. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 8 van 9 gevonden artikelen
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