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| Titel: |
Experimental intracerebral hematoma in the rat |
| Auteur: |
Barth, Alain Guzman, Raphael Andres, Robert H. Mordasini, Pasquale Barth, Laurence Widmer, Hans R. |
| Verschenen in: |
Restorative neurology and neuroscience |
| Paginering: | Jaargang 25 (2007) nr. 1 pagina's 1-7 |
| Jaar: |
2007-04-23 |
| Inhoud: |
Purpose: Current models of intracerebral hematoma are difficult to use for neurotransplantation studies because of high mortality and important variations of morphology, size and location of blood deposits. We propose a modification of the autologous blood infusion technique in rats to reduce these limitations. Methods: The modification consisted in a mechanical microlesion preceding blood infusion. A canula was stereotactically introduced into the striatum of adult rats. Subsequently, a parenchyma lesion was created by a rotating microcatheter coaxially inserted through the canula, followed by slow infusion of 30 μl autologous blood during 5 minutes. Controls included canula insertion only and canula + microlesion. Hematoma volume/morphology were quantified and the animals behaviorally analysed using standardized tests. Results: Surgical mortality was 0/54 rats. One animal died during follow-up. Hematoma volume was constant and significantly higher (15.20 ± 0.60 mm^3) than control lesions (canula: 0.11 ± 0.01 mm^3; canula + trauma: 0.51 ± 0.01 mm^3). Hematoma edges were sharply delineated and the perihematomal region histologically preserved. Rats with hematoma showed initially a reduced spontaneous rotational behaviour. They also showed persisting deficits of forelimb placing ability. Conclusions: The advantages of this model include a systematic control of all steps of hematoma production, high reproducibility of volume, size, and location of blood deposits, preservation of perihematomal brain tissue, and quantifiable neurological deficits. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
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