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Details van artikel 7 van 19 gevonden artikelen
| Titel: |
Lead Exposures Increases Oxidative Stress in Serum Deprived E14 Mesencephalic Cultures. |
| Auteur: |
Marzia Scortegagna Elsbeth Chikhale Ingeborg Hanbauer |
| Verschenen in: |
Restorative neurology and neuroscience |
| Paginering: | Jaargang 12 (2005) nr. 2-3 pagina's 95-101 |
| Jaar: |
2005-10-20 |
| Inhoud: |
E14 mesencephalic cultures grown 6 days in Neurobasal Medium containing 10% horse serum consist of differentiated neurons and astroglia. In these cultures, glutathione and metallothionein-I/II are enriched in astrocytes and play an important role in heavy metal scavenging and oxidative stress response. A 24 h exposure to 25 µM Pb, in serum-containing medium, elevated the glutathione content by more than twofold and increased the metallothionein I/II-immunolabeled protein band. In contrast, exposure to 3 to 25 µM Pb is serum-free medium increased Pb uptake by cells 2 to 4-times above the levels found in 10% serum-containing medium, reduced the glutathione level and obliterated the metallothionein-I/II protein band. The rapid decrease of metallothionein-I/II and glutathione levels in serum-free medium implies that their regulation may depend on a serum factor operative in inducing immediate early genes. Exposure to 6 µM Pb in serum-free or in B27-supplemented medium increased the carbonyl content of several protein bands above control levels indicating that under conditions that curtail metallothionein induction Pb exposure causes increased oxidative stress. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 7 van 19 gevonden artikelen
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