Analysis of Bioaerosols from Chicken Houses by Culture and Non-Culture Method
Titel:
Analysis of Bioaerosols from Chicken Houses by Culture and Non-Culture Method
Auteur:
Chi, Miao-Ching Li, Chih-Shan
Verschenen in:
Aerosol science and technology
Paginering:
Jaargang 40 (2006) nr. 12 pagina's 1071-1079
Jaar:
2006-12
Inhoud:
In livestock breeding, high bioaerosol concentrations in environments such as chicken houses are an occupational health concern. Two sampling methods (impinger and filter) and three non-culture methods [epifluoresence microscopy with fluorochrome (EFM/FL), flow cytometry with the fluorochrome (FCM/FL), and fluorescent in situ hybridization (FISH)] were used to monitor the total concentration, viability, and culturability of bioaerosols in chicken houses. These results were compared to the commonly used culture method. Total microbial cell concentrations measured by the non-culture methods averaged about 5 × 107 cells/m3. However, culture method underestimated bioaerosol concentrations by a factor of 10. For sampler comparison, viability determined following impinger collection was higher than that following filter method. In conclusion, impinger was considered an appropriate sampler, and EFM/FL, FCM/FL, and FISH approaches could adequately assess total microbial cell concentration and viability of bioaerosols in environmental samples.
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