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| Titel: |
Microprocessor-controlled freezing device for cryopreservation of cell samples |
| Auteur: |
Danko Djurić Vujo Drndarević Dragana Vujić |
| Verschenen in: |
Bio-medical materials and engineering |
| Paginering: | Jaargang 9 (2001) nr. 3 pagina's 171-178 |
| Jaar: |
2001-04-01 |
| Inhoud: |
A freezing device for cryopreservation of blood mononuclear cells has been developed. The device is microcontroller operated, allowing cell freezing by a fully automatic, unattended process. To ensure optimum preservation, the temperature in the cell suspension uniformly decreases from room temperature to -100^{\circ}C and then the samples are transferred to long-term storage. The performance of the device has been tested using both physiological solution and a sample of cell suspension. The control of temperature variation of cell suspension in the entire temperature range has been realised with an accuracy better than \pm0.1%. The viability of cells recovered from the frozen samples was 95%. The nitrogen consumption for one cycle of cryopreservation was 1.5l. In addition to the fully automatic mode, the manual and semi-automatic modes are available for research purposes. The device has been designed using low cost and widely used electronic components and materials, it is compact and simple to operate. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 5 van 7 gevonden artikelen
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