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| Titel: |
Analysis of human mesenchymal stem cells on a cross-linked collagen-based surgical implant material |
| Auteur: |
Arca, Turkan Proffitt, Joanne Genever, Paul |
| Verschenen in: |
Bio-medical materials and engineering |
| Paginering: | Jaargang 22 (2012) nr. 5 pagina's 261-276 |
| Jaar: |
2012-09-28 |
| Inhoud: |
Biomimetic scaffolds, delivered to the patient with or without cell loading, can offer improved treatments for a range of damaged or diseased tissues. In this work we evaluated the capacity of a cross-linked collagen-based implant material (Permacol™) to support the survival and growth of multipotent stromal cells or mesenchymal stem cells (MSCs) for potential applications in soft-tissue reconstruction and compared it to uncross-linked collagen. We showed, using confocal imaging, histology, quantitative analysis of seeding efficiencies by the MTT assay and Live/Dead analysis, that Permacol™ supported the adherence, growth and survival of MSCs preserving a healthy cell phenotype. MSCs infiltrated both matrices, although migration rates were significantly higher on uncross-linked matrices for some donors. Our work demonstrates that Permacol™ could maintain human MSCs in long-term culture supporting its use as a cell-seeded biomaterial for durable and strong repair in tissue reconstruction. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
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