|
| << vorige | volgende >> |
Tijdschrift beschrijving
Alle jaargangen van het bijbehorende tijdschrift
Alle afleveringen van het bijbehorende jaargang
Alle artikelen van de bijbehorende aflevering
Details van artikel 4 van 10 gevonden artikelen
| Titel: |
Gene expression analyses of human macrophage phagocytizing sub-μ titanium particles by allergy DNA chip (GenopalTM) |
| Auteur: |
Taira, Masayuki Nezu, Takashi Sasaki, Minoru Kimura, Shigenobu Kagiya, Tadayoshi Harada, Hidemitsu Narushima, Takayuki Araki, Yoshima |
| Verschenen in: |
Bio-medical materials and engineering |
| Paginering: | Jaargang 19 (2009) nr. 1 pagina's 63-70 |
| Jaar: |
2009-05-20 |
| Inhoud: |
The purpose of this study was to examine gene expressions of macrophage phagocytizing sub-μ Ti particles by a DNA chip. Human monocytic cell line THP-1 was differentiated into macrophages by culturing for two days in medium supplemented with 200 nM phorbol ester (PMA). The macrophages were then cultured in four media: medium without PMA (control); medium with suspended sub-μ Ti particles (0.5 wt%); medium with 1.0 μg/ml lipopolysaccharide (LPS); and medium with LPS and Ti particles. After 6 hours' culture, total RNA were extracted and gene expressions were evaluated by DNA allergy chip with 205 allergy and inflammation related gene spots. We found that phagocytosis of sub-μ Ti particles and LPS independently and synergistically up-regulated 17 inflammation-related genes more than two-fold. The extensive expressions of four genes (CCL1, IL1B, IL6 and IL8) were further confirmed by real-time quantitative PCR. It turned out that dual stimulation of LPS and Ti particles most up-regulated three genes (IL1B, IL6 and IL8), followed by LPS while Ti particles moderately but least increased, suggesting that phagocytosis of sub-μ Ti particles induces moderate inflammation with its degree less than LPS, but phagocytosis of sub-μ Ti particles has the potential to worsen inflammation caused by LPS-stimulated macrophages. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 4 van 10 gevonden artikelen
| << vorige | volgende >> |