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| Titel: |
Culture medium study of human mesenchymal stem cells for practical use of tissue engineering and regenerative medicine |
| Auteur: |
Nakamura, Sayaka Yamada, Yoichi Baba, Shunsuke Kato, Harumi Kogami, Hiroyuki Takao, Makoto Matsumoto, Naoyuki Ueda, Minoru |
| Verschenen in: |
Bio-medical materials and engineering |
| Paginering: | Jaargang 18 (2008) nr. 3 pagina's 129-136 |
| Jaar: |
2008-08-13 |
| Inhoud: |
Mesenchymal stem cells (MSCs) are multipotent cells that have potential to differentiate into various phenotypes and appear useful for therapeutic applications in regenerative medicine. Mesenchymal Stem Cell Basal Medium (MSCBM; Cambrex®) is a widespread and suitable medium used in MSC cultivation, but it is extremely difficult to use generally for clinical treatment because of its unclear traceability and cost. Assessment of cost-effectiveness is a critical issue for successful practical application; therefore, we have evaluated the effects of a generally used medium, Dulbecco's Modified Eagle's Medium (D-MEM) on the expansion of MSCs in comparison with MSCBM. To isolate human MSCs, bone marrow aspirates were taken and cultured in MSCBM or D-MEM. Proliferation assay indicated that MSCs isolated in both media showed a similar growth rate. When supplemented with osteo-inductive reagents, alkaline phosphatase activity was not significantly different between cells in D-MEM and MSCBM. Moreover, the cells expressed identical mesenchymal lineage markers, but not endothelial and hematopoietic lineage markers. Our findings suggest that cells obtained from bone marrow and cultured in D-MEM might possess proliferative capacity and the potential to differentiate into an osteogenic lineage. In conclusion, D-MEM might be a suitable basal medium for the cultivation of MSCs for clinical applications. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 2 van 6 gevonden artikelen
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