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| Titel: |
Validation of a viral and bacterial inactivation step during the extraction and purification process of porcine collagen |
| Auteur: |
Forest, P. Morfin, F. Bergeron, E. Dore, J. Bensa, S. Wittmann, C. Picot, S. Renaud, F.N.R. Freney, J. Gagnieu, C. |
| Verschenen in: |
Bio-medical materials and engineering |
| Paginering: | Jaargang 17 (2007) nr. 4 pagina's 199-208 |
| Jaar: |
2007-06-29 |
| Inhoud: |
In the last few years, regulations for biomolecule production, and especially for extraction and purification of animal molecules such as collagen, have been reinforced to ensure the sanitary safety of the materials. To be authorized to market biomaterials based on collagen, manufacturers now have to prove that at least one step of their process is described in guidelines to inactivate prion, viruses, and bacteria. The present study focuses on the inactivation step performed during the extraction and purification of porcine type I atelocollagen. We chose to determine the reduction factor of a 1 M NaOH step on porcine parvovirus and four bacterial strains inactivation. During the extraction step, we deliberately inoculated the collagen suspension with the different microorganisms tested. Then, 1 M NaOH was added to the suspension for 1 hour at 20°C. We demonstrated that this treatment totally inactivated S. aureus, P. aeruginosa, C. albicans and A. niger which are bacterial strains responsible of severe human pathology. The reduction factors reached more than 4 logs for B. cereus spores and 4 logs for the porcine parvovirus. are encouraging as those two microorganisms are known to be very resistant to inactivation. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 8 van 8 gevonden artikelen
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