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| Titel: |
Evaluation of chondrocytes expression embedded in thermoresponsive poly(amino acid)s with sol–gel transition |
| Auteur: |
Chueh, Shang-kai Tachibana, Yoichi Uyama, Hiroshi Kobayashi, Shiro Tomita, Naohide |
| Verschenen in: |
Bio-medical materials and engineering |
| Paginering: | Jaargang 17 (2007) nr. 3 pagina's 137-146 |
| Jaar: |
2007-05-04 |
| Inhoud: |
Poly(N-substituted α/β-asparagine) was evaluated as a thermoresponsible and an injectable scaffold for cartilage regeneration. Solutions of this polymer are liquid state below 25°C and nonfluid hydrogel above 35°C, allowing an aqueous solution containing cells at room temperature to form a hydrogel with encapsulated cells at physiological body temperature. Chondrocytes were isolated from joint of 4-week-old Japanese white rabbits, dispersed within the thermoresponsive polymer solution and maintained for up to 72 hours in vitro. The polymer solutions demonstrated concentration-dependent inhibitory effect on chondrocytes multiplication. After the three-day cultivation, the survival rate of the chondrocytes fell into a 70~90% ranges among all the tested polymer concentrations. The morphology studies showed that there were some physical and/or chemical stress leading cells to necrosis and some extent apoptosis. Some physical and/or chemical stress may be applied, and over 70% of the chondrocytes could survive through the stress, suggesting that some phenotype could have been selected from the heterogeneous mixture of chondrocytes. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 4 van 7 gevonden artikelen
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