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| Titel: |
Development of an optimised culture medium for keratocytes in monolayer |
| Auteur: |
Builles, Nicolas Bechetoille, Nicolas Justin, Virginie Ducerf, Amélie Auxenfans, Céline Burillon, Carole Sergent, Michèle Damour, Odile |
| Verschenen in: |
Bio-medical materials and engineering |
| Paginering: | Jaargang 16 (2006) nr. supplement-4 pagina's S95-S104 |
| Jaar: |
2006-07-13 |
| Inhoud: |
Our objective was to formulate a medium for monolayer culture optimising both keratocyte growth and preservation of the keratocyte phenotype. Methods: An experimental matrix selected 14 media to test, using 7 components. Selection criteria were growth rates over 5 passages and expression of the CD34 marker. Results: Acetylcholine, insulin and vitamin C had no effect on growth and differentiation. The DMEM + Ham F12 1 : 1 based medium was selected for its initial effect on growth. At concentrations of 5 ng/ml, b-FGF improved the percentage of CD34+ cells without reducing growth rates. New-born calf serum (NCS) had a greater effect on growth than foetal calf serum (FCS). We showed three major interactions: between b-FGF and IGF-1, FCS and IGF-1 and NCS and b-FGF. Conclusion: We selected the following medium, which provides optimal growth and preservation of the CD34+ phenotype: DMEM/HAM-F12 + 10% NCS + 5 ng/ml b-FGF + antibiotics. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 5 van 17 gevonden artikelen
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