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| Titel: |
Use of allogenic epidermal sheets for difficult wound healing: Selection and testing of relevant growth factors |
| Auteur: |
Auxenfans, C. Colloud, M. Debard, A.L. Braye, F.M. Amini, M. Allombert-Blaise, V. Builles, N. Claudy, A. Damour, O. |
| Verschenen in: |
Bio-medical materials and engineering |
| Paginering: | Jaargang 16 (2006) nr. supplement-4 pagina's S73-S83 |
| Jaar: |
2006-07-13 |
| Inhoud: |
The clinical interest of using allogenic epidermal sheets (AES) has largely been shown [1,2,3]. As well as covering, they also stimulate healing, by simultaneously secreting numerous growth factors (GFs), although little is known on their mechanism of action. Our objectives were to: (a) devise a test for the efficacy of AES release, (b) select keratinocyte-secreting strains and optimal culture conditions. Three GFs were selected: IL-1α, IL-8 and VEGF. Three different keratinocyte strains were cultured for 3 and 6 days after confluence for 3 passages. Assays were performed after 3 h and 24 h+3 h after dispase treatment (AES conservation for 24 h then change of medium and sampling after 3 h). AES were found to secrete GFs in DMEM and the amounts were greater when cultured for 6 rather than 3 days after confluence. Each strain had different secretory patterns depending on passage and time in culture, this variability being explained by inter-individual heterogeneity. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 17 van 17 gevonden artikelen
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