Digital Library
Close Browse articles from a journal
 
<< previous    next >>
     Journal description
       All volumes of the corresponding journal
         All issues of the corresponding volume
           All articles of the corresponding issues
                                       Details for article 7 of 8 found articles
 
 
  Skin cell culture on an ear-shaped scaffold created by fused deposition modelling
 
 
Title: Skin cell culture on an ear-shaped scaffold created by fused deposition modelling
Author: H. Cai
G. Azangwe
D.E.T. Shepherd
Appeared in: Bio-medical materials and engineering
Paging: Volume 15 (2005) nr. 5 pages 375-380
Year: 2005-09-19
Contents: Tissue engineering, where cells attach and grow on a scaffold, has the potential to produce replacement ears made from natural tissues and replace the need for rubber prosthetic ears. This study investigated the feasibility of using the rapid prototyping technique of Fused Deposition Modelling (FDM) to produce an ear-shaped scaffold. A three-dimensional image of the ear was used to manufacture ear-shaped scaffolds from ABS (acrylonitrile/butadiene/styrene) plastic using FDM. Human dermal fibroblasts were seeded on the scaffold (coated with fibronectin) to attach and grow in culture medium in an incubator for two weeks. Human keratinocytes were then seeded on to the fibroblast layer to attempt to produce a more realistic skin covering. The morphology of the cells were observed using scanning electron microscopy. The results show that a realistic ear-shaped scaffold can be made using FDM. Human fibroblasts were found to attach and grow. Human keratinocytes were successfully attached and grown on top of the fibroblasts and this resulted in a skin covering over the scaffold. This study shows that FDM has great potential as a manufacturing technique for ear-shaped scaffolds for tissue engineering.
Publisher: IOS Press
Source file: Elektronische Wetenschappelijke Tijdschriften
 
 

                             Details for article 7 of 8 found articles
 
<< previous    next >>
 
 Koninklijke Bibliotheek - National Library of the Netherlands