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                                       Details van artikel 6 van 12 gevonden artikelen
 
 
  Patterned layers of adsorbed extracellular matrix proteins: influence on mammalian cell adhesion
 
 
Titel: Patterned layers of adsorbed extracellular matrix proteins: influence on mammalian cell adhesion
Auteur: C.C. Dupont-Gillain
J.A. Alaerts
J.L. Dewez
P.G. Rouxhet
Verschenen in: Bio-medical materials and engineering
Paginering: Jaargang 14 (2004) nr. 3 pagina's 281-291
Jaar: 2004-07-26
Inhoud: Three patterned systems aiming at the control of mammalian cell behavior are presented. The determinant feature common to these systems is the spatial distribution of extracellular matrix (ECM) proteins (mainly collagen) on polymer substrates. This distribution differs from one system to another with respect to the scale at which it is affected, from the supracellular to the supramolecular scale, and with respect to the way it is produced. In the first system, the surface of polystyrene was oxidized selectively to form micrometer-scale patterns, using photolithography. Adsorption of ECM proteins in presence of a competitor was enhanced on the oxidized domains, allowing selective cell adhesion to be achieved. In the second system, electron beam lithography was used to engrave grooves (depth and width ∼1 μm) on a poly(methyl methacrylate) (PMMA) substratum. No modification of the surface chemistry associated to the created topography could be detected. Cell orientation along the grooves was only observed when collagen was preadsorbed on the substratum. In the third system, collagen adsorbed on PMMA was dried in conditions ensuring the formation of a nanometer-scale pattern. Cell adhesion was enhanced on such patterned collagen layers compared to smooth collagen layers.
Uitgever: IOS Press
Bronbestand: Elektronische Wetenschappelijke Tijdschriften
 
 

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